Year: 2018 | Month: April | Volume 11 | Issue 2
Production and Optimization of an Alkaline Protease from Acinetobacter variabilis Isolated from Soil Samples
Abstract:
Protease enzymes have immense commercial value and play a pivotal role in application of various industrial sectors. Microbial proteases are one of the important groups of industrially and commercially produced enzymes which have several applications. In this study 148 bacterial strains were isolated from 50 different soil samples of slaughter house, fish market and sewage wastes of Lucknow, Uttar Pradesh, India. Out of which thirty eight strains competent of secreting extracellular alkaline protease. In preliminary screening the isolate SSB2 showed highest ability to hydrolyzed casein and skimmed milk which was done on skim milk agar media. Based on biochemical test the isolate showed positive for casein, starch, catalase and negative for gram staining, indole, methyl red, voges proskauer, gelatin, urea, oxidase, hemolysis and triple sugar iron test and found to be non motile. Strain SSB2 with the maximum yield alkaline protease was identified as Acinetobacter variabilis based on nucleotide homology and phylogenetic analysis (16S rDNA sequencing). Protease production was enhanced by optimizing the culture conditions. Many physical parameters were studied to optimize the maximum yield of alkaline protease. The maximum enzyme activity were observed with optimum incubation time, temperature, pH, carbon, nitrogen sources, NaCl and metallic ions were determined as 36 h, 37°C, pH 11.0, 1% glucose, 1% yeast extract, 1M NaCl, and 1mM Zn2+, respectively for protease production. The study revealed that the strain of A. variabilis is a potent source of alkaline protease. In consequence, such additions can supplement alkaline protease production and their application in various industries.
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